Long Distance PELDOR Measurements on the Histone Core Particle
نویسندگان
چکیده
منابع مشابه
Long distance PELDOR measurements on the histone core particle.
The nucleosome core particle is the fundamental unit of chromatin structure and at its heart is the histone core octamer composed of histones H4, H3, H2A and H2B. To understand the structure dynamics and function of chromatin it is important to be able to probe the structures of its component parts in a variety of ways. Site directed spin-labeling technology has enabled the insertion of nitroxi...
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Crystallographic and NMR approaches have provided a wealth of structural information about protein domains. However, often these domains are found as components of larger multi domain polypeptides or complexes. Orienting domains within such contexts can provide powerful new insight into their function. The combination of site specific spin labelling and Pulsed Electron Double Resonance (PELDOR)...
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The chromatin core particle DNA conformation deduced in broad outline by Finch et al. [Finch, J. T., Lutter, L. C., Rhodes, D., Brown, R. S., Rushton, B., Levitt, M. & Klug, A. (1977) Nature 269, 29-36] can be described in detail using other available experimental results. Histone binding sites compatible with the pattern of pancreatic DNase I digestion (Simpson, R. T. & Whitlock, J. P., Jr. (1...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2009
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja807918f